Protoplast formation and cell wall regeneration in Clostridium perfringens
نویسندگان
چکیده
منابع مشابه
Protoplast formation and cell wall regeneration in Clostridium perfringens.
A protocol was developed for the efficient production and regeneration of Clostridium perfringens protoplasts. Cell wall regeneration frequencies of up to 5% were obtained.
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Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1985
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.50.4.1097-1099.1985